Прегледај по Аутор "Škrbić, Ranko"
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- СтавкаCOMPARATIVE POSTOPERATIVE ANALGESIA WITH FEMORAL NERVE BLOCK ”3-IN-1” AND WITH FASCIA ILIACA COMPARTMENT NERVE BLOCK AFTER HIP ALLOARTHROPLASTY(Serbian Medical Society, Section Kragujevac, 2016) Lončar Stojiljković, Dragana; Stojiljković, Miloš P.; Golijanin, Ranko; Novaković Bursać, Snježana; Škrbić, RankoObjective. Hip arthroplasty is one of the most common operations in elderly population. Pre- and postoperative risks increase their postoperative morbidity and mortality. One of the most important factors, which is included in the perioperative risks, is pain. Control of postoperative pain diminishes the incidence and severity of complications afterwards. Methods. We compared three different techniques of pain control in 30 patients scheduled for elective hip arthroplasty: 1) pharmacological – morphine 5 or 10 mg iv q6h, depending on body weight, paracetamol 1 g i.v. q6h (MP), 2) nerve block: femoral nerve block, single shot (FNB) and 3) fascia iliaca compartment nerve block (FICNB). Measurement of pain intensity was performed with numerical pain scale (NPS). Systolic blood pressure and consumption of additional analgesics on demand were monitored, as well as the duration of nerve blocks. Results. Both nerve blocks produced significantly lower pain scores than the purely pharmacological approach (MP 5.4+0.6 vs. FNB 2.8+1.6 and FICNB 2.9+1.2 after the first postoperative hour). Consequently, the first group required more additional morphine and paracetamol after the first hour compared to FNB and FICNB groups. Morphine was significantly more frequently added in the MP group (3.4+0.4 mg/kg IV) than in the FNB (2.1+0.5 mg/kg i.v.) and FICNB (2.5+0.4 mg/kg i.v.). Maximal duration of analgesia was 5 hours in MP group compared to 9 and 8 hours after FNB and FICNB, respectively. Conclusion. Postoperative analgesia with blocks enables better pain control, better cardiovascular stability and less adverse effect than the classical morphine-based analgesia.
- СтавкаEffect of simvastatin on proinflammatory cytokines production during lipopolysaccharide-induced inflammation in rats(Slovak Academy of Sciences, 2009) Nežić, Lana; Škrbić, Ranko; Dobrić, Silva; Stojiljković, Miloš P.; Šatara, Svjetlana S.; Milovanović, Zoran A.; Stojaković, NatašaThe effect of simvastatin applied in a short-term pretreatment on proinflammatory cytokines production in acute systemic inflammation induced by endotoxin – lipopolysaccharide (LPS) in rats was investigated. Both LPS and simvastatin doses were established in separate experiments in which increasing doses of both compounds were given to obtain the LD50 LPS and the maximally protective dose of simvastatin against LD50 LPS. To determine the anti-inflammatory effect, simvastatin was given orally for 5 days, followed by a single intraperitoneal non-lethal dose of LPS (0.25 LD50). Plasma concentrations of tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β and IL-6 were measured by enzyme-linked immunosorbent assay. The acute i.p. LD50 LPS amounted to 22.15 mg/kg. Simvastatin of 20 mg/kg p.o. was maximally protective against LD50 LPS, and this dose was used for studying its effects on LPS-induced cytokines production. Cytokines concentrations were significantly increased upon challenge of non-lethal dose of LPS. The peak levels of TNF-α and IL-1β were significantly suppressed by simvastatin, compared to control rats only treated with dimethylsulfoxide before LPS. In contrast, simvastatin did not affect IL-6 levels at all timepoints. Simvastatin pretreatment given orally produced acute anti-inflammatory effects by inhibiting TNF-α and IL-1β, but no IL-6 production.
- СтавкаImmunomodulatory Properties of Pomegranate Peel Extract in a Model of Human Peripheral Blood Mononuclear Cell Culture(MDPI, 2022) Čolić, Miodrag; Bekić, Marina; Tomić, Sergej; Đokić, Jelena; Radojević, Dušan; Ševikin, Katarina; Miljuš, Nataša; Marković, Milan; Škrbić, RankoPomegranate peel extract (PoPEx) has been shown to have antioxidant and anti-inflammatory properties, but its effect on the adaptive immune system has not been sufficiently investigated. In this study, the treatment of human peripheral blood mononuclear cells (PBMC) with PoPEx (range 6.25–400 g/mL) resulted in cytotoxicity at concentrations of 100 g/mL and higher, due to the induction of apoptosis and oxidative stress, whereas autophagy was reduced. At non-cytotoxic concentrations, the opposite effect on these processes was observed simultaneously with the inhibition of PHA-induced PBMC proliferation and a significant decrease in the expression of CD4. PoPEx differently modulated the expression of activation markers (CD69, CD25, ICOS) and PD1 (inhibitory marker), depending on the dose and T-cell subsets. PoPEx (starting from 12.5 g/mL) suppressed the production of Th1 (IFN-), Th17 (IL-17A, IL-17F, and IL-22), Th9 (IL-9), and proinflammatory cytokines (TNF- and IL-6) in culture supernatants. Lower concentrations upregulated Th2 (IL-5 and IL-13) and Treg (IL-10) responses as well as CD4+CD25hiFoxp3+ cell frequency. Higher concentrations of PoPEx increased the frequency of IL-10- and TGF- -producing T-cells (much higher in the CD4+ subset). In conclusion, our study suggested for the first time complex immunoregulatory effects of PoPEx on T cells, which could assist in the suppression of chronic inflammatory and autoimmune diseases.
- СтавкаPomegranate Peel Extract Differently Modulates Gene Expression in Gingiva-Derived Mesenchymal Stromal Cells under Physiological and Inflammatory Conditions(MDPI, 2023) Čolić, Miodrag; Miljuš, Nataša; Ðokić, Jelena; Bekić, Marina; Krivokuća, Aleksandra; Tomić, Sergej; Radojević, Dušan; Radanović, Marina; Eraković, Mile; Ismaili, Bashkim; Škrbić, RankoPomegranate has shown a favorable effect on gingivitis/periodontitis, but the mechanisms involved are poorly understood. The aim of this study was to test the effect of pomegranate peel extract (PoPEx) on gingiva-derived mesenchymal stromal cells (GMSCs) under physiological and inflammatory conditions. GMSC lines from healthy (H) and periodontitis (P) gingiva (n = 3 of each) were established. The lines were treated with two non-toxic concentrations of PoPEX (low—10; high—40 g/mL), with or without additional lipopolysaccharide (LPS) stimulation. Twenty-four genes in GMSCs involved in different functions were examined using real-time polymerase chain reaction (RT-PCR). PoPEx (mostly at higher concentrations) inhibited the basal expression of IL-6, MCP-1, GRO-a, RANTES, IP-10, HIF-1a, SDF-1, and HGF but increased the expression of IL-8, TLR3, TGF-b, TGF-b/LAP ratio, IDO-1, and IGFB4 genes in H-GMSCs. PoPEx increased IL-6, RANTES, MMP3, and BMP2 but inhibited TLR2 and GRO-a gene expression in P-GMSCs. LPS upregulated genes for proinflammatory cytokines and chemokines, tissue regeneration/repair (MMP3, IGFBP4, HGF), and immunomodulation (IP-10, RANTES, IDO-1, TLR3, COX-2), more strongly in P-GMSCs. PoPEx also potentiated most genes’ expression in LPS-stimulated P-GMSCs, including upregulation of osteoblastic genes (RUNX2, BMP2, COL1A1, and OPG), simultaneously inhibiting cell proliferation. In conclusion, the modulatory effects of PoPEx on gene expression in GMSCs are complex and dependent on applied concentrations, GMSC type, and LPS stimulation. Generally, the effect is more pronounced in inflammation-simulating conditions.
- СтавкаSeroprevalence of SARS-CoV-2 antibodies among primary healthcare workers in the Republic of Srpska, Bosnia & Herzegovina: A cross-sectional study(Academy Kiado, Budapest, 2022) Knežević, Darija; Petković, Miroslav; Božić, Ljiljana; Miljuš, Nataša; Mijović, Biljana; Aćimović, Jela; Djaković-Dević, Jelena; Puhalo-Sladoje, Dragana; Mašić, Srđan; Spaić, Dragan; Todorović, Nevena; Pilipović-Broćeta, Nataša; Petrović, Verica; Bokonjić, Dejan; Stojiljković, Miloš P.; Škrbić, RankoHealthcare workers (HCW) in primary healthcare centres in the Republic of Srpska, Bosnia and Herzegovina, are on the first combat line with COVID-19. This study aimed to assess the seroprevalence of SARS-CoV-2 among HCW at the primary healthcare centres and to analyse the risk exposure to COVID-19, clinical signs and vaccination status. A cross-sectional study was conducted among HCW at the selected primary healthcare centres between 19 March and 30 April 2021. Antibodies against the SARS-CoV-2 virus were detected by enzyme-linked immunosorbent assay (ELISA). A total of 1,023 HCW (mean age 45 years; 71% female) were included in the study. The anti-SARS-CoV-2 antibodies were detected in 69.5% of all participants. There was a significant difference in seropositivity among primary healthcare centres from different geographical regions. As many as 432 (42%) of all participants had confirmed COVID-19 symptoms before the study and, 84.8% of them were seropositive. This study showed that 702 primary HCW were vaccinated with any of these vaccines: Sputnik V, Sinopharm, Pfizer/Biontech. High titre of SARS-CoV-2 antibodies was found amongst those who received one (92.6%) or both (97.2%) doses of vaccines. In this study, we report high prevalence of SARS-CoV-2 antibody among HCW in primary healthcare in the Republic of Srpska, Bosnia and Herzegovina during the third pandemic wave.
- СтавкаSeroprevalence of SARS-CoV-2 antibodies and knowledge, attitude and practice toward COVID-19 in the Republic of Srpska-Bosnia & Herzegovina: A population-based study(PLOS, 2022) Mijović, Biljana; Mašić, Srđan; Petković, Miroslav; Knežević, Darija; Aćimović, Jela; Djaković-Dević, Jelena; Puhalo-Sladoje, Dragana; Zeljković, Branislav; Spaić, Dragan; Bokonjić, Dejan; Škrbić, RankoThe aim of the study was to assess the seroprevalence of SARS-CoV-2 in the Republic of Srpska, Bosnia and Herzegovina, and to analyse the knowledge, attitudes and practices of the population toward COVID-19. This population-based study was conducted in a group of 1,855 randomly selected individuals from all municipalities from 1 December 2020 to 15 January 2021. All individuals were asked to sign a consent form and to fill in a questionnaire, following which a blood samples were collected. Total anti-SARS-CoV-2 antibodies were determined in serum specimens using the total Ab ELISA assay. The overall seroprevalence rate was 40.3%. Subjects aged <65 years were 2.06 times more likely to be seropositive than those aged �65, and 30% of seropositive individuals presented no COVID-19 symptoms. The household members of seropositive individuals were 2.24 times more likely to develop COVID-19 symptoms than the household members of seronegative individuals. More than 95% of respondents believe that preventive measures are very important to control the infection transmission. Majority of respondents wear the masks properly, maintain the required physical distance whenever possible and wash hands with soap. Nearly 50% of individuals were of the opinion that the vaccine could prevent the infection. This study showed that an overall SARS-CoV-2 seropositivity rate by the middle of January 2021 was very high. Attitudes and practices regarding the COVID-19 indicate that additional efforts should be taken in order to improve the health education with a focus on preventive measures and vaccination.
- СтавкаSimvastatin and Indomethacin Have Similar Anti-Inflammatory Activity in a Rat Model of Acute Local Inflammation(Wiley, 2009) Nežić, Lana; Škrbić, Ranko; Dobrić, Silva; Stojiljković, Miloš P.; Jaćević, Vesna; Stoisavljević Šatara, Svjetlana; Milovanović, Zoran A.; Stojaković, NatašaStatins, such as simvastatin, lower circulating cholesterol levels and are widely prescribed for the treatment of hypercholesterolaemia. Several studies have shown unexpected effects of statins on inflammation. We studied the anti-inflammatory effect of simvastatin using a standard model of an acute local inflammation, the carrageenan-induced footpad oedema. Experimental groups (n=6–8) were given simvastatin in a dose range 5–30 mg/kg, indomethacin 1–8 mg/kg and methylcellulose (control)per os. Footpad volume was measured with a plethysmograph and compared with the pre-injection volume of the same paw. Swelling (in microlitres) was then calculated, and in drug-treated animals, per cent inhibition was derived through comparison with the control group. Histopathological examination of the skin biopsies was performed to examine severity of paw skin lesions and to confirm the simvastatin-induced inhibition of acute inflammation. Both simvastatin and indomethacin administered orally, 1 hr before carrageenan injection, significantly reduced the extent of footpad oedema. Indomethacin dose-dependently blocked the swelling; the maximal effect was obtained with 8 mg/kg by 48.3% (P<0.05). Simvastatin produced a comparable anti-inflammatory activity at a dose of 5 mg/kg (32%), while 10 and 30 mg/kg caused a 47.6% and 51.7% reduction, respectively, with the maximal effect observed at 20 mg/kg by 57.2% (P<0.05). The comparison of the ED50 of these agents on molar basis showed equipotent anti-inflammatory activity. Histopathological examination of the footpad skin biopsies revealed that simvastatin, dose-dependently and comparablly to indomethacin, reduced polymorphonuclear leucocyte infiltration. These data support the hypothesis that simvastatin has an acute anti-inflammatory activity.
- СтавкаThe Effect of Three-Month Vitamin D Supplementation on the Levels of Homocysteine Metabolism Markers and Inflammatory Cytokines in Sera of Psoriatic Patients(MDPI, 2021) Prtina, Alma; Rašeta Simović, Nela; Milivojac, Tatjana; Vujnić, Milorad; Grabež, Milkica; Djuric, Dragan; Stojiljković, Miloš P.; Soldat Stanković, Valentina; Čolić, Miodrag J.; Škrbić, RankoPsoriasis is an autoimmune and inflammatory skin disease. Psoriatic patients express higher levels of plasma homocysteine (Hcy) concentration and pro-inflammatory mediators than healthy people; this is frequently associated with vitamin D deficiency. The aim of this clinical study was to investigate the effects of high doses of vitamin D supplementation on the parameters of Hcy metabolism and cytokines in sera of psoriatic patients. This prospective study was conducted on 40 psoriatic patients who had the vitamin D deficiency. All patients received vitamin D 5000 IU/day for three months. Clinical and biochemical measurements were taken at baseline and at follow up (3 months). The results showed that the severity of clinical features, measured by the psoriasis area severity index (PASI) score, were considerably improved in patients after vitamin D supplementation. After vitamin D supplementation, most of the patients (n = 25 or 62.5%) had mild clinical form (p < 0.001). After twelve weeks of intervention period, there were significant increases in vitamin D and B12 serum levels in comparison to the levels that had been measured at the beginning of the study (56.77 14.66 nmol/L and 301.08 95.02 pg/mL vs. 103.85 32.20 nmol/L and 362.81 118.56 pg/mL, respectively; p < 0.001). Moreover, serum levels of Hcy and folate were significantly lower at the end of the study in comparison with the initial levels (12.45 1.92 mol/L and 8.01 3.88 mg/mL vs. 10.38 1.66 mol/L and 6.27 2.60 mg/mL, respectively). High doses of vitamin D supplementation led to a significant decrease in pro-inflammatory cytokines (IFN-7, TNF- , IL-1 , IL-6, IL-8, and IL-17) and high-sensitivity C-reactive protein (hsCRP), whereas the production of anti-inflammatory cytokines (IL-10, IL-5) was up-regulated. In conclusion, supplementation with high doses of vitamin D could be one of the possible preventive and therapeutic measures to reduce systemic inflammation in psoriatic patients.