Прегледај по Аутор "Krunić, Jelena"
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- СтавкаBreaking the Gingival Barrier in Periodontitis(MDPI, 2023) Vitkov, Ljubomir; Singh, Jeeshan; Schauer, Christine; Minnich, Bernd; Krunić, Jelena; Oberthaler, Hannah; Gamsjaeger, Sonja; Herrmann, Martin; Knopf, Jasmin; Hannig, MatthiasThe break of the epithelial barrier of gingiva has been a subject of minor interest, albeit playing a key role in periodontal pathology, transitory bacteraemia, and subsequent systemic lowgrade inflammation (LGI). The significance of mechanically induced bacterial translocation in gingiva (e.g., via mastication and teeth brushing) has been disregarded despite the accumulated knowledge of mechanical force effects on tight junctions (TJs) and subsequent pathology in other epithelial tissues. Transitory bacteraemia is observed as a rule in gingival inflammation, but is rarely observed in clinically healthy gingiva. This implies that TJs of inflamed gingiva deteriorate, e.g., via a surplus of lipopolysaccharide (LPS), bacterial proteases, toxins, Oncostatin M (OSM), and neutrophil proteases. The inflammation-deteriorated gingival TJs rupture when exposed to physiological mechanical forces. This rupture is characterised by bacteraemia during and briefly after mastication and teeth brushing, i.e., it appears to be a dynamic process of short duration, endowed with quick repair mechanisms. In this review, we consider the bacterial, immune, and mechanical factors responsible for the increased permeability and break of the epithelial barrier of inflamed gingiva and the subsequent translocation of both viable bacteria and bacterial LPS during physiological mechanical forces, such as mastication and teeth brushing.
- СтавкаDental pulp pain in young and postmenopausal women: a pilot study(Serbian Medical Society - Dental Section, Belgrade, Serbia, 2016) Krunić, Jelena; Mladenović, Irena; Stojanović, NikolaIntroduction This pilot study was aimed to compare pulpal pain provoked by electrical and thermal (cold) stimuli in healthy young women during various phases of menstrual cycle and postmenopausal women. Material and methods The study included 20 regularly menstruating healthy women and 20 postmenopausal women. Electrical (electrical pulp tester) and cold (refrigerant spray) stimuli were performed on mandibular central incisors, twice in regularly menstruating (menstrual and luteal phases) and once in postmenopausal women. Results were expressed as pain threshold values for electrical pulp stimulation (0-80 units) and pain intensity scores (visual numeric scale, from 0 to 10) for cold stimulation. Results In young women, higher pain electrical threshold (p=0.484) and pain sensitivity score (p=0.015) were observed in luteal in comparison to menstrual phase. In postmenopausal women, electrical pain threshold was significantly higher while pain intensity score was significantly lower than in young women, regardless of the menstrual phase and painful stimuli. Conclusion Lower responsiveness to dental pulp pain was obtained in young women in luteal phase and postmenopausal women
- СтавкаEvaluation of permeability of root dentin after different irrigation protocols(Serbian Medical Association, 2018) Bjelović, Ljiljana; Krunić, Jelena; Stojanović, Nikola; Erić, Jelena; Kanjevac, TatjanaIntroduction/Objective This study was aimed at evaluating dentin permeability after irrigation with sodium hypochlorite (NaOCl) and final rinse with chlorhexidine (CHX), ethylenediamine tetraacetic acid (EDTA) + CHX, and new combination products: QMiX or MTAD. Methods Roots of 60 maxillary incisors were randomly divided into five groups (n = 12) before instrumentation and irrigation with NaOCl according to the final irrigation regimen: CHX (2% CHX), EDTA + CHX (17% EDTA + 2% CHX), QMiX, MTAD, and control group (distilled water). After final irrigation, ten roots of each group were horizontally sectioned and dye penetration was evaluated in the coronal, middle, and apical thirds. Remaining samples were subjected to scanning electron microscopy. Data were analyzed with ANOVA/Tukey’s test. Results Less dye penetration was found in CHX group compared with control as well as with QMiX and MTAD group in all thirds (p < 0.05). A significant difference between the control and EDTA + CHX, QMiX or MTAD group was observed only in the apical root third (p < 0.05). Conclusion Dentin permeability was significantly reduced after final irrigation with CHX, but not after use of other final irrigation solutions, except in the apical third of the root canal.
- СтавкаInfluence of different forms of calcium hydroxide and chlorhexidine intracanal medicaments on the outcome of endodontic treatment of teeth with chronic apical periodontitis(Serbian Medical Association, Belgrade, 2018) Stojanović, Nikola; Krunić, Jelena; Mladenović, Irena; Stojanović, Zorica; Apostolska, Sonja; Živković, SlavoljubIntroduction/Objective The aim of this study was to determine clinical and radiographic periapical healing of teeth with apical periodontitis treated with different formulations of calcium hydroxide (CH) – paste (CH-paste) and gutta-percha points (CH-GP) – as well as those of chlorhexidine (CHX) – gel (CHXgel) and gutta-percha points (CHX-GP) –12 months after therapy. Methods Eighty patients with chronic apical periodontitis were randomly allocated to four treatment groups according to the intracanal medicament used: CH-paste, CH-GP, CHX-gel, and CHX-GP group. Seventy-eight patients were analyzed clinically and radiographically 12 months postoperatively. The periapical index (PAI) was used for the radiographic evaluation of treatment. Results Overall outcome was classified according to radiographic evaluation only, since clinical success was observed in all the patients. In all the groups, significant reduction in PAI scores was observed (p < 0.001). The proportions of healed teeth (PAI ≤ 2) were 73.7%, 60%, 68.4%, and 65% in CH-paste, CH-GP, CHX-gel and CHX-GP group, respectively, with no significant differences between the groups. Conclusion The results suggest that there are no differences between investigated CH- and CHX-delivery systems regarding treatment outcome of teeth with apical periodontitis.
- СтавкаPeriodontal Disease in Young Adults as a Risk Factor for Subclinical Atherosclerosis: A Clinical, Biochemical and Immunological Study(MDPI, 2023) Cicmil, Smiljka; Cicmil, Ana; Pavlic, Verica; Krunić, Jelena; Sladoje Puhalo, Dragana; Bokonjić, Dejan; Čolić, MiodragAlthough a strong relationship between periodontal disease (PD) and atherosclerosis was shown in adults, little data are published in younger PD patients. Therefore, this study aimed to investigate and correlate clinical parameters of PD, pro- and immunoregulatory cytokines in gingival crevicular fluid (GCF) and serum, biochemical and hematological parameters associated with atherosclerosis risk, and carotid intima-media thickness (IMT) in our younger study participants (n = 78) (mean age 35.92 3.36 years) who were divided into two equal groups: subjects with and without PD. PD patients had higher values of IMT, hs-CRP, triglycerides, total cholesterol, and LDL; most proinflammatory and Th1/Th17-associated cytokines in GCF; and IL-8, IL-12, IL-18, and IL-17A in serum compared to subjects without PD. These cytokines in GCF positively correlated with most clinical periodontal parameters. Clinical periodontal parameters, TNF- and IL-8 in GCF and IL-17A, hs-CRP, and LDL in serum, had more significant predictive roles in developing subclinical atherosclerosis (IMT 0.75 mm) in comparison with other cytokines, fibrinogen, and other lipid status parameters. Hs-CRP correlated better with the proinflammatory cytokines than the parameters of lipid status. Except for serum IL-17A, there was no significant association of clinical and immunological PD parameters with lipid status. Overall, these results suggest that dyslipidemia and PD status seem to be independent risk factors for subclinical atherosclerosis in our younger PD population.
- СтавкаPeriodontitis-Derived Dark-NETs in Severe Covid-19(Frontiers, 2022) Vitkov, Ljubomir; Knopf, Jasmin; Krunić, Jelena; Schauer, Christine; Schoen, Janina; Minnich, Bernd; Hannig, Matthias; Herrmann, MartinThe frequent severe COVID-19 course in patients with periodontitis suggests a link of the aetiopathogenesis of both diseases. The formation of intravascular neutrophil extracellular traps (NETs) is crucial to the pathogenesis of severe COVID-19. Periodontitis is characterised by an increased level of circulating NETs, a propensity for increased NET formation, delayed NET clearance and low-grade endotoxemia (LGE). The latter has an enormous impact on innate immunity and susceptibility to infection with SARS-CoV-2. LPS binds the SARS-CoV-2 spike protein and this complex, which is more active than unbound LPS, precipitates massive NET formation. Thus, circulating NET formation is the common denominator in both COVID-19 and periodontitis and other diseases with lowgrade endotoxemia like diabetes, obesity and cardiovascular diseases (CVD) also increase the risk to develop severe COVID-19. Here we discuss the role of propensity for increased NET formation, DNase I deficiency and low-grade endotoxaemia in periodontitis as aggravating factors for the severe course of COVID-19 and possible strategies for the diminution of increased levels of circulating periodontitis-derived NETs in COVID-19 with periodontitis comorbidity.
- СтавкаPrevalence of Enterococcus faecalis and Porphyromonas gingivalis in Infected Root Canals and Their Susceptibility to Endodontic Treatment Procedures: A Molecular Study(Serbian Medical Association, 2014) Stojanović, Nikola; Krunić, Jelena; Popović, Branka; Stojičić, Sonja; Živković, SlavoljubIntroduction Because apical periodontitis is recognizably an infectious disease, elimination or reduction of intracanal bacteria is of utmost importance for optimum treatment outcome. Objective The prevalence of Enterococcus faecalis and Porphyromonas gingivalis in infected root canals was studied Also, the effect of endodontic therapy by using intracanal medicaments, calcium hydroxide paste (CH) or gutta-percha points containing calcium hydroxide (CH-GP) or chlorhexidine (CHX-GP) on these microorganisms was assessed by polymerase chain reaction (PCR) assay. Methods Fifty-one patients with chronic apical periodontitis were randomly allocated in one of the following groups according to the intracanal medicament used: CH, CH-GP and CHX-GP group. Bacterial samples were taken upon access (S1), after chemomechanical instrumentation (S2) and after 15-day medication (S3). PCR assay was used to detect the presence of selected bacteria. Results E. faecalis was detected in 49% (25/51) and P. gingivalis in 17.6% (9/51) of the samples. Samples which showed no bacterial presence at S1 were excluded from further analysis. Overall analysis of all 29 samples revealed significant differences between S1 and S2 (p<0.001), S2 and S3 (p<0.05), and S1 and S3 (p<0.001). When distinction was made between the intracanal medications, there was a significant difference in the number of PCR positive samples between S1 and S2, S1 and S3, but not between S2 and S3 samples. Conclusion E. faecalis is more prevalent than P. gingivalis in primary endodontic infection. Intracanal medication in conduction with instrumentation and irrigation efficiently eliminates E. faecalis and P. gingivalis from infected root canals.
- СтавкаPsychometric properties of the Child Oral Impacts on Daily Performances (C‑OIDP) index: a cross‑sectional and an intervention study of adolescents in Bosnia and Herzegovina(Springer, 2023) Erić, Jelena; Bjelović, Ljiljana; Janković, Svjetlana; Davidović, Bojana; Bozović, Djordje; Krunić, JelenaBackground The clinical measures are not sufficient to assess oral health because they don’t tell us anything about functional and psychosocial aspects of oral health and do not reflect person’s concerns and subjectively perceived symptoms. This study aimed to investigate the validity, reliability and responsiveness of the child Oral Impacts on Daily Performances (C-OIDP) index among Bosnian 12-14 years old schoolchildren. Methods The study population comprised 203 primary schoolchildren aged 12-14 years attending three schools in the eastern part of Bosnia and Herzegovina. Data were collected through: a clinical oral examination, oral health questionnaire and C-OIDP questionnaire. The validity and reliability of the C-OIDP were tested on a sample of 203 school-going children while responsiveness of the C-OIDP was assessed on 42 randomly chosen participants requiring a dental treatment. Results In terms of reliability, Cronbach’s alpha coefficient and the intraclass correlation coefficient were 0.86 and 0.85, respectively. Regarding the testing of construct validity, the C-OIDP score was increased as children’s selfreported oral health changed from excellent to very bad and from very satisfied to dissatisfied. There was a significant improvement in C-OIDP post-treatment score compared with C-OIDP pre-treatment score. Overall, 63.4% of participants reported at least one oral impact in the last 3 months. The most affected performances were “eating” (38.4%) and “speaking” (25.1%). Conclusion The Bosnian version of the C-OIDP showed satisfactory validity, reliability and responsiveness and can be used as an appropriate OHRQoL measure for further epidemiological researches.
- СтавкаSalivary flow rate and decayed, missing, and filled teeth (DMFT) in female patients with schizophrenia on chlorpromazine therapy(Elsevier, 2013) Krunić, Jelena; Stojanović, Nikola; Ivković, Nedeljka; Stojić, DragicaBackground/purpose: The purpose of the study was to investigate relationship between saliva flow rates, estrogen levels, and caries prevalence in female psychiatric patients under antipsychotic therapy. Materials and methods: Sixty-one institutionalized psychiatric females (31 patients treated with chlorpromazine only and 30 patients treated with chlorpromazine and biperiden) were compared with 36 unmedicated healthy females. The unstimulated whole saliva (UWS) flow rate and serum estrogen were measured. Caries prevalence was recorded in terms of decayed, missing, and filled teeth (DMFT). Results: The UWS flow rate in the control group was 0.35 0.18 mL/min and the DMFT 18.8 5.7. In comparison, UWS flow rates were 0.25 0.15 mL/min (P Z 0.003) and 0.07 0.05 mL/min (PZ 0.000) in patients on chlorpromazine and patients on chlorpromazine as well as biperiden, respectively, and DMFT values were 22.7 4.6 (P Z 0.003) and 26.5 5.3 (P Z 0.000), respectively. Patientson chlorpromazinewithamenorrheahad reducedUWS flowrate and estrogenlevels with respect to controls (PZ0.036; PZ0.000, respectively). Correlation analysis revealed significant correlations betweenUWS flow rate and DT, DMFT, number of used drugs and estrogen level. Conclusion: It seems that chlorpromazine-induced hyposalivation included (apart from its antimuscarinic effect) a neuroendocrine effect which affected the estrogen levels.
- СтавкаVesicular Messages from Dental Biofilms for Neutrophils(MDPI, 2024) Vitkov, Ljubomir; Krunić, Jelena; Dudek, Johanna; Reddy Bobbili, Madhusudhan; Grillari, Johannes; Hausegger, Bernhard; Mladenović, Irena; Stojanović, Nikola; Dietrich Krautgartner, Wolf; Oberthaler, Hannah; Schauer, Christine; Herrmann, Martin; Singh, Jeeshan; Minnich, Bernd; Hannig, MatthiasThe encounter between dental biofilm and neutrophils in periodontitis remains elusive, although it apparently plays a crucial role in the periodontal pathology and constitutes a key topic of periodontology. Dental biofilm and neutrophils were isolated from orally healthy persons and patients with periodontitis. We investigated biofilm and its particle-shedding phenomenon with electron microscopy and nanoparticle tracking analysis (NTA); biofilm shedding–neutrophil interactions were examined ex vivo with epi-fluorescence microscopy. For this purpose, we used acellular dental biofilm shedding, purified lipopolysaccharide (LPS), and phorbol 12-myristate 13-acetate (PMA) as activators, and the interleukin 8 receptor beta (CXCR2) inhibitor and the anti-interleukin 8 receptor alpha (CXCR1) antibody as modulators. The shedding of acellular dental biofilms overwhelmingly consists of bacterial extracellular vesicles (BEVs). The latter induced the moderate formation of neutrophil extracellular traps (NETs) in orally healthy subjects and a strong formation in patients with periodontitis. A CXCR2 inhibitor and an anti-CXCR1 antibody had a minor effect on NET formation. Neutrophils from patients with periodontitis exhibited NET hyper-responsiveness. BEVs were stronger inducers of NET formation than purified LPS and PMA. A plateau of neutrophil responsiveness is reached above the age of 40 years, indicating the abrupt switch of maladaptive trained immunity (TI) into the activated modus. Our results suggest that dental biofilms consist of and disseminate immense amounts of outer membrane vesicles (OMVs), which initiate NET formation via a non-canonical cytosolic LPS/caspase-4/11/Gasdermin D pathway. This modus of NET formation is independent of neutrophil elastase (NE), myeloperoxidase (MPO), peptidylarginine deiminase 4 (PAD4), and toll-like receptors (TLR). In periodontitis, the hyper-responsiveness of neutrophils to BEVs and the increased NET formation appear to be a consequence of TI.