Mesenchymal Stromal Cells from Healthy and Inflamed Human Gingiva Respond Differently to Porphyromonas gingivalis

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dc.citation.spage3510
dc.citation.volume23
dc.contributor.authorBekić, Marina
dc.contributor.authorRadanović, Marina
dc.contributor.authorĐokić, Jelena
dc.contributor.authorTomić, Sergej
dc.contributor.authorEraković, Mile
dc.contributor.authorRadojević, Dušan
dc.contributor.authorDuka, Miloš
dc.contributor.authorMarković, Dejan
dc.contributor.authorMarković Milan
dc.contributor.authorIsmaili, Bashkim
dc.contributor.authorBokonjić, Dejan
dc.contributor.authorČolić, Miodrag
dc.date.accessioned2023-05-16T09:52:16Z
dc.date.available2023-05-16T09:52:16Z
dc.date.issued2022
dc.description.abstractGingiva-Derived Mesenchymal Stromal Cells (GMSCs) have been shown to play an important role in periodontitis. However, how P. gingivalis, one of the key etiological agents of the disease, affects healthy (H)- and periodontitis (P)-GMSCs is unknown. To address this problem, we established 10 H-GMSC and 12 P-GMSC lines. No significant differences in morphology, differentiation into chondroblasts and adipocytes, expression of characteristic MSCS markers, including pericyte antigens NG2 and PDGFR, were observed between H- and P-GMSC lines. However, proliferation, cell size and osteogenic potential were higher in P-GMSCs, in contrast to their lower ability to suppress mononuclear cell proliferation. P. gingivalis up-regulated the mRNA expression of IL-6, IL-8, MCP-1, GRO- , RANTES, TLR-2, HIF-1 , OPG, MMP-3, SDF-1, HGF and IP-10 in P-GMSCs, whereas only IL-6, MCP-1 and GRO- were up-regulated in H-GMSCs. The expression of MCP-1, RANTES, IP-10 and HGF was significantly higher in P-GMSCs compared to H-GMSCs, but IDO1 was lower. No significant changes in the expression of TLR-3, TLR-4, TGF- , LAP, IGFBP4 and TIMP-1 were observed in both types of GMSCs. In conclusion, our results suggest that P-GMSCs retain their pro-inflammatory properties in culture, exhibit lower immunosuppressive potential than their healthy counterparts, and impaired regeneration-associated gene induction in culture. All these functions are potentiated significantly by P. gingivalis treatment.
dc.identifier.doi10.3390/ijms23073510
dc.identifier.urihttps://vaseljena.ues.rs.ba/handle/123456789/150
dc.language.isoen
dc.publisherMDPI
dc.sourceMolecular Sciences
dc.subjectGingiva-DerivedMesenchymal Stromal Cells; Porphyromonas gingivalis; cell differentiation; gene expression; inflammation; tissue regeneration
dc.titleMesenchymal Stromal Cells from Healthy and Inflamed Human Gingiva Respond Differently to Porphyromonas gingivalis
dc.typeArticle
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