Effects of protamine sulphate on spontaneous and calcium-induced contractile activity in the rat uterus are potassium channels-mediated

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dc.citation.epage148
dc.citation.spage143
dc.citation.volume28
dc.contributor.authorOreščanin-Dušić, Zorana
dc.contributor.authorMilovanović, Slobodan
dc.contributor.authorRadojičić, Ratko
dc.contributor.authorNikolić-Kokić, Aleksandra
dc.contributor.authorAppiah, Isabella
dc.contributor.authorSlavić, Marija
dc.contributor.authorČutura, Neđo
dc.contributor.authorTrbojević, Stevan
dc.contributor.authorSpasić, Mihajlo
dc.contributor.authorBlagojević, Duško
dc.date.accessioned2023-09-18T06:58:46Z
dc.date.available2023-09-18T06:58:46Z
dc.date.issued2009
dc.description.abstractProtamine sulphate (PS) effect on spontaneous and calcium-induced rhythmic contractions of isolated virgin rat uteri was studied. PS caused dose-dependent relaxation of both types of contractions (two-way ANOVA, significant dose effects). Pretreatment with NG-nitro-L-arginine methyl ester (L-NAME; 10–5 mol/l), methylene blue (MB; 0.9 × 10–6 mol/l) or propranolol (1.7 × 10–5 mol/l) enhanced PS-mediated uterine muscle relaxation of spontaneous contractions. Dosedependent relaxation of spontaneous active isolated rat uterus with PS was lower in uteri pretreated with single dose of tetraethylammonium (TEA; 6 × 10–3 mol/l), glibenclamide (2 × 10–6 mol/l) and 4-aminopyridine (4-AP; 10–3 mol/l). Calcium-induced activity of the isolated rat uterus pretreated with the same concentration of L-NAME, MB, or propranolol modified the kinetic of PS-induced relaxation without changes in EC50 values. Pre-treatment with glibenclamide, TEA and 4-AP significantly reduce PS relaxing effect of calcium-induced activity and according to EC50 values the order of magnitude was glibenclamide > TEA > 4-AP. PS is mixture of polyamines and may activate different signal-transduction pathways. Our results cleary demonstrate that in uterine smooth muscle PS act dominantly through potassium chanels and marginaly through β-adrenergic receptos or nitric oxide-dependent pathways.
dc.identifier.urihttps://vaseljena.ues.rs.ba/handle/123456789/785
dc.language.isoen
dc.publisherSlovak Academy of Sciences
dc.sourceGeneral Physiology and Biophysics
dc.subjectNitric oxide, Protamine sulphate, Potassium channels, Rat uterus
dc.titleEffects of protamine sulphate on spontaneous and calcium-induced contractile activity in the rat uterus are potassium channels-mediated
dc.typeArticle
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