Pomegranate Peel Extract Differently Modulates Gene Expression in Gingiva-Derived Mesenchymal Stromal Cells under Physiological and Inflammatory Conditions

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dc.citation.spage15407
dc.citation.volume24
dc.contributor.authorČolić, Miodrag
dc.contributor.authorMiljuš, Nataša
dc.contributor.authorÐokić, Jelena
dc.contributor.authorBekić, Marina
dc.contributor.authorKrivokuća, Aleksandra
dc.contributor.authorTomić, Sergej
dc.contributor.authorRadojević, Dušan
dc.contributor.authorRadanović, Marina
dc.contributor.authorEraković, Mile
dc.contributor.authorIsmaili, Bashkim
dc.contributor.authorŠkrbić, Ranko
dc.date.accessioned2024-12-05T11:32:40Z
dc.date.available2024-12-05T11:32:40Z
dc.date.issued2023
dc.description.abstractPomegranate has shown a favorable effect on gingivitis/periodontitis, but the mechanisms involved are poorly understood. The aim of this study was to test the effect of pomegranate peel extract (PoPEx) on gingiva-derived mesenchymal stromal cells (GMSCs) under physiological and inflammatory conditions. GMSC lines from healthy (H) and periodontitis (P) gingiva (n = 3 of each) were established. The lines were treated with two non-toxic concentrations of PoPEX (low—10; high—40 g/mL), with or without additional lipopolysaccharide (LPS) stimulation. Twenty-four genes in GMSCs involved in different functions were examined using real-time polymerase chain reaction (RT-PCR). PoPEx (mostly at higher concentrations) inhibited the basal expression of IL-6, MCP-1, GRO-a, RANTES, IP-10, HIF-1a, SDF-1, and HGF but increased the expression of IL-8, TLR3, TGF-b, TGF-b/LAP ratio, IDO-1, and IGFB4 genes in H-GMSCs. PoPEx increased IL-6, RANTES, MMP3, and BMP2 but inhibited TLR2 and GRO-a gene expression in P-GMSCs. LPS upregulated genes for proinflammatory cytokines and chemokines, tissue regeneration/repair (MMP3, IGFBP4, HGF), and immunomodulation (IP-10, RANTES, IDO-1, TLR3, COX-2), more strongly in P-GMSCs. PoPEx also potentiated most genes’ expression in LPS-stimulated P-GMSCs, including upregulation of osteoblastic genes (RUNX2, BMP2, COL1A1, and OPG), simultaneously inhibiting cell proliferation. In conclusion, the modulatory effects of PoPEx on gene expression in GMSCs are complex and dependent on applied concentrations, GMSC type, and LPS stimulation. Generally, the effect is more pronounced in inflammation-simulating conditions.
dc.identifier.doi10.3390/ijms242015407
dc.identifier.urihttps://vaseljena.ues.rs.ba/handle/123456789/1378
dc.language.isoen
dc.publisherMDPI
dc.sourceInternational Journal of Molecular Sciences
dc.subjectgingiva; mesenchymal stromal cells; pomegranate; periodontitis; gene expression
dc.titlePomegranate Peel Extract Differently Modulates Gene Expression in Gingiva-Derived Mesenchymal Stromal Cells under Physiological and Inflammatory Conditions
dc.typeArticle
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