Quality Testing of Industrially Produced Essential Oil of Fir (Abies alba L.) from the Republic of Srpska

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Taylor & Francis
The quality of the essential oil obtained from the wood verdure of fir from the territory of the Republic of Srpska was analyzed by examining the chemical composition, antioxidant activity and antimicrobial activity of the samples produced by the process of hydrodistillation in industrial production conditions. Two samples of fir essential oil were tested, depending on how long the plant material stood before the hydrodistillation process. The chemical composition of the essential oil was analyzed using GC/MS and GC/FID analysis. The antioxidant and antimicrobial activity of fir essential oil has been examined for its possible application as a natural antioxidant and antimicrobial agent. The obtained results prove the presence of 50 components in the first analyzed sample and 44 components in the second analyzed sample. The total components present (98.3% and 98.7%) were identified. The most common are monoterpene hydrocarbons, followed by sesquiterpene hydrocarbons and oxygen-containing monoterpenes. The antioxidant activity was investigated by the DPPH assay. Fir essential oil has shown the best antioxidant properties after 120 minutes of incubation with an EC50 value of 32.0 ± 0.03 mg/ml and 29.93 ± 0.08 mg/ml. Antimicrobial activity was investigated using a disk diffusion method. The results showed large zones of inhibition for both tested oil samples and for all eight bacterial strains analyzed (from 14.33 ± 0.58 mm to 16.67 ± 1.15 mm) as well as for the fungal strain. The chemical composition, antioxidant and antimicrobial action of fir essential oil obtained from plant material from the Republic of Srpska indicate significant phytomedicine potency.
Кључне речи
Essential oil, silver fir, GC/MS analysis, antioxidant activity, antimicrobial activity.