Halogenated Boroxine K2[B3O3F4OH] Modulates Metabolic Phenotype and Autophagy in Human Bladder Carcinoma 5637 Cell Line

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dc.citation.spage2919
dc.citation.volume29
dc.contributor.authorElez-Burnjaković, Nikolina
dc.contributor.authorPojskić, Lejla
dc.contributor.authorHaverić, Anja
dc.contributor.authorLojo-Kadrić, Naida
dc.contributor.authorHadžić Omanović, Maida
dc.contributor.authorSmajlović, Ajla
dc.contributor.authorKalaydjiev, Svetoslav
dc.contributor.authorMaksimović, Milka
dc.contributor.authorJoksimović, Bojan
dc.contributor.authorHaverić, Sanin
dc.date.accessioned2024-11-12T13:32:16Z
dc.date.available2024-11-12T13:32:16Z
dc.date.issued2024
dc.description.abstractHalogenated boroxine K2[B3O3F4OH] (HB), an inorganic derivative of cyclic anhydride of boronic acid, is patented as a boron-containing compound with potential for the treatment of both benign and malignant skin changes. HB has effectively inhibited the growth of several carcinoma cell lines. Because of the growing interest in autophagy induction as a therapeutic approach in bladder carcinoma (BC), we aimed to assess the effects of HB on metabolic phenotype and autophagy levels in 5637 human bladder carcinoma cells (BC). Cytotoxicity was evaluated using the alamar blue assay, and the degree of autophagy was determined microscopically. Mitochondrial respiration and glycolysis were measured simultaneously. The relative expression of autophagy-related genes BECN1, P62, BCL-2, and DRAM1 was determined by real-time PCR. HB affected cell growth, while starvation significantly increased the level of autophagy in the positive control compared to the basal level of autophagy in the untreated negative control. In HB-treated cultures, the degree of autophagy was higher compared to the basal level, and metabolic phenotypes were altered; both glycolysis and oxidative phosphorylation (OXPHOS) were decreased by HB at 0.2 and 0.4 mg/mL. Gene expression was deregulated towards autophagy induction and expansion. In conclusion, HB disrupted the bioenergetic metabolism and reduced the intracellular survival potential of BC cells. Further molecular studies are needed to confirm these findings and investigate their applicative potential
dc.identifier.doi10.3390/molecules29122919
dc.identifier.urihttps://vaseljena.ues.rs.ba/handle/123456789/1285
dc.language.isoen
dc.publisherMDPI
dc.sourceMolecules
dc.subjectcytotoxicity; autophagy; BC cells; gene expression; metabolic phenotype
dc.titleHalogenated Boroxine K2[B3O3F4OH] Modulates Metabolic Phenotype and Autophagy in Human Bladder Carcinoma 5637 Cell Line
dc.typeArticle
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