Association of vdr, cyp27b1, cyp24a1 and mthfr gene polymorphisms with oral lichen planus risk
Датум
2016
Наслов журнала
Журнал ISSN
Наслов волумена
Издавач
Springer
Апстракт
Objectives
The current study investigated the association between VDR EcoRV (rs4516035), FokI (rs2228570), ApaI (rs7975232) and TaqI (rs731236), CYP27B1 (rs4646536), CYP24A1 (rs2296241), and MTHFR (rs1801133) gene polymorphisms and risk of oral lichen planus (OLP) occurrence.
Materials and methods
The study group consisted of 65 oral lichen planus patients and 100 healthy blood donors in the control group. Single nucleotide polymorphisms were genotyped by real time PCR or PCR-restriction fragment length polymorphism (RFLP) method.
Results
Heterozygous as well as mutated genotype of vitamin D receptor (VDR) FokI (rs2228570) polymorphism was associated with increased oral lichen planus risk in comparison with wild type genotype (odds ratio (OR) = 3.877, p = 0.017, OR = 38.153, p = 0.001, respectively). A significantly decreased OLP risk was observed for heterozygous genotype of rs2296241 polymorphism in CYP24A1 gene compared with the wild type form (OR = 0.314, p = 0.012). VDR gene polymorphisms ApaI and TaqI were in linkage disequilibrium (D’ = 0.71, r 2 = 0.22). Identified haplotype AT was associated with decreased OLP risk (OR = 0.592, p = 0.047).
Conclusion
Our results highlight the possible important role of VDR FokI (rs2228570) and CYP24A1 rs2296241 gene polymorphisms for oral lichen planus susceptibility.
Clinical relevance
Identification of new molecular biomarkers could potentially contribute to determination of individuals with OLP predisposition.
Опис
Кључне речи
Oral lichen planus .VDR . CYP27B1 . CYP24A1 .MTHFR . Single nucleotide polymorphisms