Прегледај по Аутор "Ismaili, Bashkim"

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    Mesenchymal Stromal Cells from Healthy and Inflamed Human Gingiva Respond Differently to Porphyromonas gingivalis
    (MDPI, 2022) Bekić, Marina; Radanović, Marina; Đokić, Jelena; Tomić, Sergej; Eraković, Mile; Radojević, Dušan; Duka, Miloš; Marković, Dejan; Marković Milan; Ismaili, Bashkim; Bokonjić, Dejan; Čolić, Miodrag
    Gingiva-Derived Mesenchymal Stromal Cells (GMSCs) have been shown to play an important role in periodontitis. However, how P. gingivalis, one of the key etiological agents of the disease, affects healthy (H)- and periodontitis (P)-GMSCs is unknown. To address this problem, we established 10 H-GMSC and 12 P-GMSC lines. No significant differences in morphology, differentiation into chondroblasts and adipocytes, expression of characteristic MSCS markers, including pericyte antigens NG2 and PDGFR, were observed between H- and P-GMSC lines. However, proliferation, cell size and osteogenic potential were higher in P-GMSCs, in contrast to their lower ability to suppress mononuclear cell proliferation. P. gingivalis up-regulated the mRNA expression of IL-6, IL-8, MCP-1, GRO- , RANTES, TLR-2, HIF-1 , OPG, MMP-3, SDF-1, HGF and IP-10 in P-GMSCs, whereas only IL-6, MCP-1 and GRO- were up-regulated in H-GMSCs. The expression of MCP-1, RANTES, IP-10 and HGF was significantly higher in P-GMSCs compared to H-GMSCs, but IDO1 was lower. No significant changes in the expression of TLR-3, TLR-4, TGF- , LAP, IGFBP4 and TIMP-1 were observed in both types of GMSCs. In conclusion, our results suggest that P-GMSCs retain their pro-inflammatory properties in culture, exhibit lower immunosuppressive potential than their healthy counterparts, and impaired regeneration-associated gene induction in culture. All these functions are potentiated significantly by P. gingivalis treatment.
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    SHORT-TERM LOW-LEVEL LASER THERAPY ATTENUATES INFLAMMATION AND PRODUCTION OF INTERLEUKIN-1, BUT ELEVATES THE LEVEL OF MATRIX METALLOPROTEINASE 9 IN CHRONIC PERIODONTITIS
    (Ektodermal Displazi Grubu, 2014) Ismaili, Bashkim; Bokonjic, Dejan
    Background / Purpose: Low- level laser therapy (LLLT) has been used as an adjunct to the treatment of chronic periodontitis (CP), but its efficacy has not been well-documented. Therefore, the aim of this study was to investigate the effect of LLLT on clinical parameters in CP patients and their relationship with concentrations of biohumoral markers of inflammation in gingival crevicular fluid (GCF). . Materials and methods: Thirty-six patients were randomly assigned to control and experimental groups after scaling. The experimental group was treated with a diode laser (wavelenght: 635 nm; power density: 100 mW/cm2), by applying the laser beam to diseased teeth for 9 days, whereas the control group did not receive LLLT. Clinical examination was performed at baseline and 10 days after the treatment. GCF samples were collected from the same periodontal site before and after therapy. The levels of interleukin 1 (IL-1) and matrix metalloproteinase 9 (MMP-9) in GCF were measured by ELISA. Results: LLLT decreased clinical parameters of CP. The levels of IL-1α and IL-1β in GCF were decreased (p< 0.05), but the level of MMP-9 was increased (p<0.01). After LLLT, the level of IL-1α correlated positively with MMP-9 (p< 0.05) and the MMP-9 levels correlated negatively with plaque index (p< 0.05) and papillary bleeding index (p< 0.01). Conclusion: LLLT attenuated periodontal inflammation in CP patients, as judged by clinical parameters and decreased levels of IL-1 in GCF. It remains to be studied whether elevated levels of MMP-9 in GCF might be beneficial for reparation processes.