Прегледај по Аутор "Kurćubić, Vladimir"

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    ENDOCRINE AND METABOLIC STATUS IN DAIRY COWS DURING TRANSITION PEROD AND MID LACTATION
    (Lithuanian Veterinary Academy, 2015) Djoković, Radojica; Kurćubić, Vladimir; Ilić, Zoran; Cincović, Marko; Petrović, Milun; Lalović, Miroslav; Jašović, Boban
    The objective of the present study was to evaluate the endocrine and metabolic changes in Simmental dairy cows during the transition period and mid lactation. Fifteen late pregnant cows, 15 early lactation cows and 15 mid lactation cows were chosen for the analysis. Blood samples were collected to measure growth hormone (GH), insulin, triiodothyronine (T3) and thyroxine (T4) by ELISA methods and beta-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), glucose, triglycerides (TG), total protein (TP), albumin and urea by different colorimetric techniques. Early lactation cows were found to have higher blood serum concentrations of GH (P < 0.05), NEFA (P < 0.05) and BHB (P < 0.05) and lower blood serum concentrations of insulin (P > 0.05), T3 (P < 0.05), T4 (P > 0.05) , glucose (P < 0.05), TG (P < 0.05), albumin (P < 0.05) and urea (P < 0.05) compared to late pregnant and mid lactation cows. Correlation analysis showed that GH levels were negatively correlated with insulin levels (r=-0.44; P < 0.05) and positively with NEFA levels (r=0.32; P < 0.05). Insulin levels negatively correlated with NEFA levels (r=-0.34; P < 0.05) and positively with T3 levels (r=0.35; P < 0.05). BHB was negatively correlated with glucose (r=-0.47; P < 0.05) and TG (r=-0.36; P < 0.05) levels (r=-0.44; P < 0.05) and positively with NEFA levels (r= 0.39; P < 0.05). These endocrine and metabolic changes can serve as useful indicators in evaluating the endocrine and metabolic status of dairy cows during lactation.
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    Endocrine and Metabolic Status of Dairy Cows during Transition Period
    (Chulalongkorn University, Thailand, 2014) Djoković, Radojica; Cincović, Marko; Kurćubić, Vladimir; Petrović, Milun; Lalović, Miroslav; Jašović, Boban; Stanimirovic, Zoran
    The objective of this study was to determine blood levels of cortisol, insulin, triiodothyronine (T3), thyroxine (T4), glucose, non-esterified fatty acids (NEFA), β-hydroxybutyrate (BHB), triglycerides (TG) and total cholesterol in dairy Holstein cows (n = 40) during the transition period. The test cows were classified into four groups: Group A (n = 10), clinically healthy late-pregnant cows from day 20 to day 10 before calving. Group B (n = 10), clinically healthy late-pregnant cows from day 10 to day 1 before calving. Group C (n = 10), clinically healthy puerperal cows. Group D (n = 10), puerperal ketotic cows. The blood serum of ketotic cows was found to have lower levels of T3 (p < 0.01), T4 (p < 0.05), insulin (p > 0.05), cortisol (p > 0.05), glucose (p < 0.01), TG (p <0.01) and total cholesterol (p < 0.05), and higher (p < 0.01) levels of NEFA and BHB, as compared to the blood levels in healthy pregnant and healthy puerperal cows. Results suggest that ketotic cows undergo homeorhetic adaptation of the regulation of organic nutrient metabolism being manifested through a decrease in the blood levels of the test hormones, resulting in increased lipomobilization, hypoglycemia, and intensive ketogenesis and lipogenesis in liver cells.