Прегледај по Аутор "Stojiljković, Miloš P."
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- СтавкаAntidotal effect of combinations obidoxime/HI-6 and memantine in mice poisoned with soman, dichlorvos or heptenophos(Ministry of Defance, Serbia, 2011) Antonijević, Biljana; Stojiljković, Miloš P.; Bokonjić, Dubravko; Vučinić, SlavicaIn acute organophosphate poisoning the issue of special concern is the appearance of muscle fasciculations and convulsions that cannot be adequately antagonised by the use of atropine and oxime therapy. The aim of this study was to examine atidotal effect of obidoxime or HI-6 combinations with memantine in mice poisoned with soman, dichlorvos or heptenophos. Methods. Male Albino mice were pretreated intravenously (iv) with increasing doses of oximes and/or memantine (10 mg/kg) at various times before poisoning with 1.3 LD-50 of soman, dichlorvos or heptenophos, in order to determine the median effective dose and the efficacy half-time. In a separate experiment, cerebral extravasation of Evans blue dye (40 mg/kg iv) was examined after application of memantine (10 mg/kg iv), midazolam (2.5 mg/kg intraperitonealy – ip) and ketamine (20 mg/kg ip) 5 minutes before soman (1 LD-50 subcutaneously – sc). Results. Coadministration of memantine induced a significant decrease in median effective dose in null time of both HI-6 (7.96 vs 1.79 moL/kg in soman poisoning) and obidoxime (16.80 vs 2.75 moL/kg in dichlorvos poisoning; 21.56 vs 6.63 moL/kg in heptenophos poisoning). Memantine and midazolam succeded to counteract the soman-induced proconvulsive activity. Conclusion. Memantine potentiated the antidotal effect of HI-6 against a lethal dose of soman, as well as the ability of obidoxime to antagonize the toxic effects of dichlorvos and heptenophos probably partly due to its anticonvulsive properties
- СтавкаCOMPARATIVE POSTOPERATIVE ANALGESIA WITH FEMORAL NERVE BLOCK ”3-IN-1” AND WITH FASCIA ILIACA COMPARTMENT NERVE BLOCK AFTER HIP ALLOARTHROPLASTY(Serbian Medical Society, Section Kragujevac, 2016) Lončar Stojiljković, Dragana; Stojiljković, Miloš P.; Golijanin, Ranko; Novaković Bursać, Snježana; Škrbić, RankoObjective. Hip arthroplasty is one of the most common operations in elderly population. Pre- and postoperative risks increase their postoperative morbidity and mortality. One of the most important factors, which is included in the perioperative risks, is pain. Control of postoperative pain diminishes the incidence and severity of complications afterwards. Methods. We compared three different techniques of pain control in 30 patients scheduled for elective hip arthroplasty: 1) pharmacological – morphine 5 or 10 mg iv q6h, depending on body weight, paracetamol 1 g i.v. q6h (MP), 2) nerve block: femoral nerve block, single shot (FNB) and 3) fascia iliaca compartment nerve block (FICNB). Measurement of pain intensity was performed with numerical pain scale (NPS). Systolic blood pressure and consumption of additional analgesics on demand were monitored, as well as the duration of nerve blocks. Results. Both nerve blocks produced significantly lower pain scores than the purely pharmacological approach (MP 5.4+0.6 vs. FNB 2.8+1.6 and FICNB 2.9+1.2 after the first postoperative hour). Consequently, the first group required more additional morphine and paracetamol after the first hour compared to FNB and FICNB groups. Morphine was significantly more frequently added in the MP group (3.4+0.4 mg/kg IV) than in the FNB (2.1+0.5 mg/kg i.v.) and FICNB (2.5+0.4 mg/kg i.v.). Maximal duration of analgesia was 5 hours in MP group compared to 9 and 8 hours after FNB and FICNB, respectively. Conclusion. Postoperative analgesia with blocks enables better pain control, better cardiovascular stability and less adverse effect than the classical morphine-based analgesia.
- СтавкаEffect of simvastatin on proinflammatory cytokines production during lipopolysaccharide-induced inflammation in rats(Slovak Academy of Sciences, 2009) Nežić, Lana; Škrbić, Ranko; Dobrić, Silva; Stojiljković, Miloš P.; Šatara, Svjetlana S.; Milovanović, Zoran A.; Stojaković, NatašaThe effect of simvastatin applied in a short-term pretreatment on proinflammatory cytokines production in acute systemic inflammation induced by endotoxin – lipopolysaccharide (LPS) in rats was investigated. Both LPS and simvastatin doses were established in separate experiments in which increasing doses of both compounds were given to obtain the LD50 LPS and the maximally protective dose of simvastatin against LD50 LPS. To determine the anti-inflammatory effect, simvastatin was given orally for 5 days, followed by a single intraperitoneal non-lethal dose of LPS (0.25 LD50). Plasma concentrations of tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β and IL-6 were measured by enzyme-linked immunosorbent assay. The acute i.p. LD50 LPS amounted to 22.15 mg/kg. Simvastatin of 20 mg/kg p.o. was maximally protective against LD50 LPS, and this dose was used for studying its effects on LPS-induced cytokines production. Cytokines concentrations were significantly increased upon challenge of non-lethal dose of LPS. The peak levels of TNF-α and IL-1β were significantly suppressed by simvastatin, compared to control rats only treated with dimethylsulfoxide before LPS. In contrast, simvastatin did not affect IL-6 levels at all timepoints. Simvastatin pretreatment given orally produced acute anti-inflammatory effects by inhibiting TNF-α and IL-1β, but no IL-6 production.
- СтавкаSeroprevalence of SARS-CoV-2 antibodies among primary healthcare workers in the Republic of Srpska, Bosnia & Herzegovina: A cross-sectional study(Academy Kiado, Budapest, 2022) Knežević, Darija; Petković, Miroslav; Božić, Ljiljana; Miljuš, Nataša; Mijović, Biljana; Aćimović, Jela; Djaković-Dević, Jelena; Puhalo-Sladoje, Dragana; Mašić, Srđan; Spaić, Dragan; Todorović, Nevena; Pilipović-Broćeta, Nataša; Petrović, Verica; Bokonjić, Dejan; Stojiljković, Miloš P.; Škrbić, RankoHealthcare workers (HCW) in primary healthcare centres in the Republic of Srpska, Bosnia and Herzegovina, are on the first combat line with COVID-19. This study aimed to assess the seroprevalence of SARS-CoV-2 among HCW at the primary healthcare centres and to analyse the risk exposure to COVID-19, clinical signs and vaccination status. A cross-sectional study was conducted among HCW at the selected primary healthcare centres between 19 March and 30 April 2021. Antibodies against the SARS-CoV-2 virus were detected by enzyme-linked immunosorbent assay (ELISA). A total of 1,023 HCW (mean age 45 years; 71% female) were included in the study. The anti-SARS-CoV-2 antibodies were detected in 69.5% of all participants. There was a significant difference in seropositivity among primary healthcare centres from different geographical regions. As many as 432 (42%) of all participants had confirmed COVID-19 symptoms before the study and, 84.8% of them were seropositive. This study showed that 702 primary HCW were vaccinated with any of these vaccines: Sputnik V, Sinopharm, Pfizer/Biontech. High titre of SARS-CoV-2 antibodies was found amongst those who received one (92.6%) or both (97.2%) doses of vaccines. In this study, we report high prevalence of SARS-CoV-2 antibody among HCW in primary healthcare in the Republic of Srpska, Bosnia and Herzegovina during the third pandemic wave.
- СтавкаSimvastatin and Indomethacin Have Similar Anti-Inflammatory Activity in a Rat Model of Acute Local Inflammation(Wiley, 2009) Nežić, Lana; Škrbić, Ranko; Dobrić, Silva; Stojiljković, Miloš P.; Jaćević, Vesna; Stoisavljević Šatara, Svjetlana; Milovanović, Zoran A.; Stojaković, NatašaStatins, such as simvastatin, lower circulating cholesterol levels and are widely prescribed for the treatment of hypercholesterolaemia. Several studies have shown unexpected effects of statins on inflammation. We studied the anti-inflammatory effect of simvastatin using a standard model of an acute local inflammation, the carrageenan-induced footpad oedema. Experimental groups (n=6–8) were given simvastatin in a dose range 5–30 mg/kg, indomethacin 1–8 mg/kg and methylcellulose (control)per os. Footpad volume was measured with a plethysmograph and compared with the pre-injection volume of the same paw. Swelling (in microlitres) was then calculated, and in drug-treated animals, per cent inhibition was derived through comparison with the control group. Histopathological examination of the skin biopsies was performed to examine severity of paw skin lesions and to confirm the simvastatin-induced inhibition of acute inflammation. Both simvastatin and indomethacin administered orally, 1 hr before carrageenan injection, significantly reduced the extent of footpad oedema. Indomethacin dose-dependently blocked the swelling; the maximal effect was obtained with 8 mg/kg by 48.3% (P<0.05). Simvastatin produced a comparable anti-inflammatory activity at a dose of 5 mg/kg (32%), while 10 and 30 mg/kg caused a 47.6% and 51.7% reduction, respectively, with the maximal effect observed at 20 mg/kg by 57.2% (P<0.05). The comparison of the ED50 of these agents on molar basis showed equipotent anti-inflammatory activity. Histopathological examination of the footpad skin biopsies revealed that simvastatin, dose-dependently and comparablly to indomethacin, reduced polymorphonuclear leucocyte infiltration. These data support the hypothesis that simvastatin has an acute anti-inflammatory activity.
- СтавкаThe Effect of Three-Month Vitamin D Supplementation on the Levels of Homocysteine Metabolism Markers and Inflammatory Cytokines in Sera of Psoriatic Patients(MDPI, 2021) Prtina, Alma; Rašeta Simović, Nela; Milivojac, Tatjana; Vujnić, Milorad; Grabež, Milkica; Djuric, Dragan; Stojiljković, Miloš P.; Soldat Stanković, Valentina; Čolić, Miodrag J.; Škrbić, RankoPsoriasis is an autoimmune and inflammatory skin disease. Psoriatic patients express higher levels of plasma homocysteine (Hcy) concentration and pro-inflammatory mediators than healthy people; this is frequently associated with vitamin D deficiency. The aim of this clinical study was to investigate the effects of high doses of vitamin D supplementation on the parameters of Hcy metabolism and cytokines in sera of psoriatic patients. This prospective study was conducted on 40 psoriatic patients who had the vitamin D deficiency. All patients received vitamin D 5000 IU/day for three months. Clinical and biochemical measurements were taken at baseline and at follow up (3 months). The results showed that the severity of clinical features, measured by the psoriasis area severity index (PASI) score, were considerably improved in patients after vitamin D supplementation. After vitamin D supplementation, most of the patients (n = 25 or 62.5%) had mild clinical form (p < 0.001). After twelve weeks of intervention period, there were significant increases in vitamin D and B12 serum levels in comparison to the levels that had been measured at the beginning of the study (56.77 14.66 nmol/L and 301.08 95.02 pg/mL vs. 103.85 32.20 nmol/L and 362.81 118.56 pg/mL, respectively; p < 0.001). Moreover, serum levels of Hcy and folate were significantly lower at the end of the study in comparison with the initial levels (12.45 1.92 mol/L and 8.01 3.88 mg/mL vs. 10.38 1.66 mol/L and 6.27 2.60 mg/mL, respectively). High doses of vitamin D supplementation led to a significant decrease in pro-inflammatory cytokines (IFN-7, TNF- , IL-1 , IL-6, IL-8, and IL-17) and high-sensitivity C-reactive protein (hsCRP), whereas the production of anti-inflammatory cytokines (IL-10, IL-5) was up-regulated. In conclusion, supplementation with high doses of vitamin D could be one of the possible preventive and therapeutic measures to reduce systemic inflammation in psoriatic patients.