New in vitro findings about halogenated boroxine cytotoxicity and deregulation of cell death-related genes in GR-M melanoma cells

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dc.citation.epage21
dc.citation.spage16
dc.citation.volume74
dc.contributor.authorElez-Burnjaković, Nikolina
dc.contributor.authorPojskić, Lejla
dc.contributor.authorHaverić, Anja
dc.contributor.authorLojo-Kadrić, Naida
dc.contributor.authorHadžić Omanović, Maida
dc.contributor.authorRamić, Jasmin
dc.contributor.authorSmajlović, Ajla
dc.contributor.authorMaksimović, Milka
dc.contributor.authorHaverić, Sanin
dc.date.accessioned2024-11-19T13:00:54Z
dc.date.available2024-11-19T13:00:54Z
dc.date.issued2023
dc.description.abstractAnti-proliferative effects of halogenated boroxine – K2(B3O3F4OH) (HB) – have been confirmed in multiple cancer cell lines, including melanoma, but the exact mechanism of action is still unknown. This study aimed to determine its cytotoxic effects on human Caucasian melanoma (GR-M) cell growth in vitro as well as on the expression of cell death-related genes BCL-2, BECN1, DRAM1, and SQSTM1. GR-M and peripheral blood mononuclear (PBM) cells were treated with different HB concentrations and their growth inhibition and relative gene expression profiles were determined using the Alamar blue assay and real-time PCR. HB significantly inhibited cell growth of both GR-M and PBM cells but was even more effective in GR-M melanoma cells, as significant inhibition occurred at a lower HB concentration of 0.2 mg/mL. GR-M BCL-2 expression was significantly downregulated (P=0.001) at HB concentration of 0.4 mg/mL, which suggests that HB is a potent tumour growth inhibitor. At the same time, it upregulated BCL-2 expression in normal (PBM) cells, probably by activating protective mechanisms against induced cytotoxicity. In addition, all but the lowest HB concentrations significantly upregulated SQSTM1 (P=0.001) in GR-M cells. Upregulated BECN1 expression suggests early activation of autophagy at the lowest HB concentration in SQSTM1 cells and at all HB concentrations in PBM cells. Our findings clearly show HB-associated cell death and, along with previous cytotoxicity studies, reveal its promising anti-tumour potential
dc.identifier.doi10.2478/aiht-2023-74-3702
dc.identifier.urihttps://vaseljena.ues.rs.ba/handle/123456789/1312
dc.language.isoen
dc.publisherInstitute for Medical Research and Occupational Health
dc.sourceArhiv za Higijenu Rada i Toksikologiju
dc.subjectanti-proliferative effect; BCL-2; BECN1, DRAM1; human Caucasian melanoma; peripheral blood mononuclear cell; SQSTM1
dc.titleNew in vitro findings about halogenated boroxine cytotoxicity and deregulation of cell death-related genes in GR-M melanoma cells
dc.typeArticle
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