Anti-inflammatory effect of amalgam on periapical lesion cells in culture

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dc.citation.epage295
dc.citation.spage289
dc.citation.volume78
dc.contributor.authorEraković, Mile
dc.contributor.authorDuka, Miloš
dc.contributor.authorBekić, Marina
dc.contributor.authorMilanović, Marijana
dc.contributor.authorTomić, Sergej
dc.contributor.authorVučević, Dragana
dc.contributor.authorČolić, Miodrag
dc.date.accessioned2023-06-07T10:46:33Z
dc.date.available2023-06-07T10:46:33Z
dc.date.issued2021
dc.description.abstractBackground/Aim. Amalgam h as b een u sed f or y ears in dentistry, but the controversy on its adverse effects, both on local oral/dental tissues and systemic health, still exists. When used for retrograde filling in apical surgery, amalgam comes in close contact with the periapical tissue, and it is sometimes responsible for the induction of periapical lesion (PL) or its exacerbation. Therefore, the aim of the study was to examine the effect of amalgam on cytotoxicity and production of pro-inflammatory cytokine by cells isolated from PL. Methods. Conditioned medium from freshly prepared amalgam (ACM) was performed according to the ISO 10993-12 by incubating the alloy in RPMI medium (0.2 g/mL) for 3 days at 37°C. Cells were isolated from 20 human PLs after apicoectomy by collagenase/DNA-ase digestion and cultured with different dilutions of ACM. Cytotoxicity was determined by MTT assay (n = 7 cultures) and apoptosis/necrosis assays (n = 8 cultures), whereas cytokine production was measured by a Flow Cytomix Microbeads Assay (n = 8 cultures). Results. Undiluted (100%) and 75% ACM was cytotoxic due to induction of apoptosis of PL cells. Non-cytotoxic concentrations of ACM (50% and 25%) inhibited the production of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-8), concentrationdependently. Conclusion. For t he f irst t ime, o ur results showed an unexpected anti-inflammatory property of amalgam on PL cells, which could be beneficial for PL healing after apicoectomy
dc.identifier.doi10.2298/VSP190225043E
dc.identifier.urihttps://vaseljena.ues.rs.ba/handle/123456789/297
dc.language.isoen
dc.publisherMinistry of Defance, Serbia
dc.sourceVOJNOSANITETSKI PREGLED
dc.subjectdental amalgam; periapical tissue; cytokines; cytotoxicity, immunologic; inflammation; apicoectomy
dc.titleAnti-inflammatory effect of amalgam on periapical lesion cells in culture
dc.typeArticle
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