Polymorphism of κ-casein and β-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia

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dc.citation.epage205
dc.citation.spage198
dc.citation.volume66
dc.contributor.authorMaletić, Milan
dc.contributor.authorAleksić, Nevenka
dc.contributor.authorVejnović, Branislav
dc.contributor.authorNikšić, Dragan
dc.contributor.authorKulić, Milan
dc.contributor.authorĐukić, Božidar
dc.contributor.authorĆirković, Dragan
dc.date.accessioned2023-08-30T10:46:55Z
dc.date.available2023-08-30T10:46:55Z
dc.date.issued2016
dc.description.abstractThe aim of this study was to determine the distribution of κ-casein (κ-CN) and β-lactoglobulin (β-Lg) genotypes in the autochtonous (Busha) and dairy (Holstein-Friesian, HF) cattle breeds with PCR-RFLP (polymerase chain reaction - restriction fragment length polymorphism). For the amplification of κ-CN and β-Lg gene fragments specific primers were used. After digestion with specific endonucleases genotypes were determined for both genes in 18 Busha and 19 HF cows. The results showed that κ-CN gene was represented with the AA genotype in 31.58 % HF cows, AB in 52.63 % cows, whilst the genotype BB was found in 15.79 % cows only. Among the examined Busha cattle 44.44 % cows had AA genotype and 55.56 % genotype AB for κ-CN. As for β-Lg gene in HF breed, AA genotype was found in 26.31 % cows, AB in 63.16 % and BB in 10.53 % cows. In Busha cows the following genotypes were established for β-Lg gene: AA in 44.44 % cows and AB in 55.56 %, whilst BB genotype was not found. These results indicate that Busha cows had a higher presence of A allelic forms of both genes (k-CN and β-lactoglobulin) than HF cows.
dc.identifier.doi10.15567/mljekarstvo.2016.0304
dc.identifier.urihttps://vaseljena.ues.rs.ba/handle/123456789/613
dc.language.isoen
dc.publisherCroatian Dairy Association
dc.sourceMljekarstvo
dc.subjectκ-casein, β-lactoglobulin, polymorphism, PCR-RFLP, Holstein-Friesian, Busha cattle
dc.titlePolymorphism of κ-casein and β-lactoglobulin genes in Busha and Holstein Friesian dairy cows in Serbia
dc.typeArticle
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