Biocompatibility and antibiofilm activity of graphene-oxide functionalized titanium discs and collagen membranes

The aims of the study were: 1) to evaluate the effect on biofilm formation of barrier membranes and titanium surfaces coated with graphene-oxide (GO); 2) to analyze the connection between the superficial topography of the tested materials and the amount of bacterial accumulation on them and 3) to analyze the biocompatibility of GO functiona lized discs using the zebrafish model. Methods: Single species bacterial biofilms (Streptococcus oralis, Veilonella parvula, Fusobacterium nucleatum, Porphyomonas gingivalis) were grown on GO-free membranes, membranes coated with 2 and 10 μg/ml of GO, GO-free and GO-coated titanium discs. The biofilms were analyzed by determining the CFU count and by Scanning Electron Microscopy (SEM) and the materials’ topography by Atomic Force Microscopy (AFM). Zebrafish model was used to determine the materials’ toxicity and inflammatory effects.
Кључне речи
Peri-Implantitis Biofilm Dental Material Graphene oxide, Zebrafish