The role of potassium channels and calcium in the relaxation mechanism of magnesium sulfate on the isolated rat uterus

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dc.citation.epage11
dc.citation.spage5
dc.citation.volume71
dc.contributor.authorSokolović, Dragana
dc.contributor.authorDrakul, Dragana
dc.contributor.authorOreščanin Dušić,
dc.contributor.authorTatalović, Nikola
dc.contributor.authorPecelj, Milica
dc.contributor.authorMilovanović, Slobodan
dc.contributor.authorBlagojević, Duško
dc.date.accessioned2023-07-17T10:48:54Z
dc.date.available2023-07-17T10:48:54Z
dc.date.issued2019
dc.description.abstractMgSO4 is used as a tocolytic agent. It is considered to be a calcium channel antagonist, but a different mechanism of its action might be involved. The aim of this study was to examine the contribution of calcium concentrations and potassium channels in the mechanism of MgSO4-mediated uterine relaxation. Isolated uteri from female Wister rats were treated with increasing MgSO4 concentrations (0.1-30 mM). MgSO4 induced dose-dependent inhibition of spontaneous activity. Addition of Ca2+ (6 mM and 12 mM) stimulated uterine contractile activity and attenuated the inhibitory activity of MgSO4. In order to analyze the role of different subtypes of potassium channels, Ca2+-stimulated uteri were pretreated with glibenclamide (Glib), a selective ATP-sensitive potassium channel inhibitor (KATP), tetraethylammonium (TEA), a non-specific inhibitor of large conductance calcium-activated potassium channels (BKCa), and 4-aminopyridine (4-AP), a voltage-sensitive potassium channel inhibitor (Kv), at concentrations that had no effect per se. Pretreatment with 4-AP had no effect on MgSO4-mediated relaxation of Ca2+-stimulated uteri. The relaxing effect of MgSO4 was potentiated by pretreatment with glibenclamide. Pretreatment with TEA attenuated the MgSO4-mediated decrease in frequency. Our results suggest that MgSO4 acts as a general calcium antagonist that influences Ca2+-mediated potassium channels. Furthermore, it seems that MgSO4 uterine relaxation activity is partially mediated by selective ATP sensitive potassium channels, suggesting an ATP-dependent role.
dc.identifier.doi10.2298/ABS180615031S
dc.identifier.urihttps://vaseljena.ues.rs.ba/handle/123456789/468
dc.language.isoen
dc.publisherSerbian Biological Society
dc.sourceArchives of Biological SciencesArchives of Biological Sciences
dc.subjectMgSO4; uterus; K+ channels; Ca2+ channels; tocolytic
dc.titleThe role of potassium channels and calcium in the relaxation mechanism of magnesium sulfate on the isolated rat uterus
dc.typeArticle
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